The Role of IgG Subclass in Antibody-Mediated

Bethany September 11, 2020 0 Comments
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The Role of IgG Subclass in Antibody-Mediated Protection against Carbapenem-Resistant Klebsiella pneumoniae

  • Monoclonal antibodies (MAbs) have the potential to assist in the battle against multidrug-resistant bacteria such as carbapenem-resistant Klebsiella pneumoniae (CR-Kp).
  • However, the characteristics by which these antibodies (Abs) function, such as the role of antibody subclass, must be determined before such modalities can be carried from the bench to the bedside. We performed a subclass switch on anticapsular monoclonal murine IgG3 (mIgG3) hybridomas and identified and purified a murine IgG1 (mIgG1) hybridoma line through sib selection. We then compared the ability of the mIgG1 and mIgG3 antibodies to control CR-Kp sequence type 258 (ST258) infection both in vitro and in vivo.
  • We found by enzyme-limited immunosorbent assay (ELISA) and flow cytometry that mIgG3 has superior binding to the CR-Kp capsular polysaccharide (CPS) and superior agglutinating ability compared to mIgG1 The mIgG3 also, predictably, had better complement-mediated serum bactericidal activity than the mIgG1 and also promoted neutrophil-mediated killing at concentrations lower than that of the mIgG1 In contrast, the mIgG1 had marginally better activity in improving macrophage-mediated phagocytosis.
  • Comparing their activities in a pulmonary infection model with wild-type as well as neutropenic mice, both antibodies reduced organ burden in a nonlethal challenge, regardless of neutrophil status, with mIgG1 having the highest overall burden reduction in both scenarios.
  • However, at a lethal inoculum, both antibodies showed reduced efficacy in neutropenic mice, with mIgG3 retaining the most activity. These findings suggest the viability of monoclonal Ab adjunctive therapy in neutropenic patients that cannot mount their own immune response, while also providing some insight into the relative contributions of immune mediators in CR-Kp protection.IMPORTANCE Carbapenem-resistant Klebsiella pneumoniae is an urgent public health threat that causes life-threatening infections in immunocompromised hosts.
  • Its resistance to nearly all antibiotics necessitates novel strategies to treat it, including the use of monoclonal antibodies. Monoclonal antibodies are emerging as important adjuncts to traditional pharmaceuticals, and studying how they protect against specific bacteria such as Klebsiella pneumoniae is crucial to their development as effective therapies.
  • Antibody subclass is often overlooked but is a major factor in how an antibody interacts with other mediators of immunity. This paper is the first to examine how the subclass of anticapsular monoclonal antibodies can affect efficacy against CR-Kp Additionally, this work sheds light on the viability of monoclonal antibody therapy in neutropenic patients, who are most vulnerable to CR-Kp infection.
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Human Galectin-1 (LGALS1)
1-CSB-EP012882HU
  • EUR 456
  • EUR 256.8
  • EUR 1570.8
  • EUR 672
  • EUR 1047.6
  • EUR 314.4
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
  • MW: 30.6 kDa
  • Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Human Galectin-1(LGALS1) expressed in E.coli
Human Galectin-1 (LGALS1)
1-CSB-EP012882HUb1
  • EUR 456
  • EUR 256.8
  • EUR 1570.8
  • EUR 672
  • EUR 1047.6
  • EUR 314.4
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
  • MW: 19.6 kDa
  • Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Human Galectin-1(LGALS1) expressed in E.coli
Human Galectin-3(Galectin-3)ELISA Kit
YLA0216HU-48T 48T
EUR 435
Human Galectin-3(Galectin-3)ELISA Kit
YLA0216HU-96T 96T
EUR 562.5
Anti Human Galectin-3 Polyclonal Antibody
KH040 each
EUR 778.8
Description: The Anti Human Galectin-3 Polyclonal Antibody is available in Europe and for worldwide shipping via Gentaur.
Recombinant Human Galectin-1
7-00427 10µg Ask for price
Recombinant Human Galectin-1
7-00428 50µg Ask for price
Recombinant Human Galectin-1
7-00429 1mg Ask for price
Human Galectin 1 Protein
20-abx066725
  • EUR 744
  • EUR 326.4
  • EUR 2230.8
  • EUR 878.4
  • EUR 543.6
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-7 working days.
Galectin-1, human recombinant
4648-10 each
EUR 170.4
Galectin-1, human recombinant
4648-1000 each
EUR 1977.6
Galectin-1, human recombinant
4648-50 each
EUR 314.4
Human Galectin 1 ELISA kit
E01G0038-192T 192 tests
EUR 1524
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A sandwich ELISA for quantitative measurement of Human Galectin 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human Galectin 1 ELISA kit
E01G0038-48 1 plate of 48 wells
EUR 624
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A sandwich ELISA for quantitative measurement of Human Galectin 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human Galectin 1 ELISA kit
E01G0038-96 1 plate of 96 wells
EUR 822
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A sandwich ELISA for quantitative measurement of Human Galectin 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human Galectin-1 (LGALS1) Protein
abx670211-50ug 50 ug
EUR 661.2
  • Shipped within 1 week.
Anti-human Galectin-3 antibody
STJ15100163 250 µg
EUR 403.2
Description: This monoclonal antibody is for studies of antigen expression in cells and tissue sections using immunocytochemistry and immunoprecipitation
anti- Galectin-3 antibody
FNab03316 100µg
EUR 606.3
  • Recommended dilution: WB: 1:500-1:5000
  • IP: 1:200-1:2000
  • IHC: 1:20-1:200
  • IF: 1:10-1:100
  • Immunogen: lectin, galactoside-binding, soluble, 3
  • Uniprot ID: P17931
  • Research Area: Cardiovascular, Cancer, Immunology, Neuroscience, Cell Division and Prolife
  • Show more
Description: Antibody raised against Galectin-3
anti- Galectin-3 antibody
FNab03317 100µg
EUR 658.5
  • Recommended dilution: WB: 1:1000-1:5000
  • IHC: 1:50-1:200
  • IF: 1:20-1:200
  • Immunogen: lectin, galactoside-binding, soluble, 3
  • Uniprot ID: P17931
  • Research Area: Cardiovascular, Cancer, Immunology, Neuroscience, Cell Division and Proliferation
Description: Antibody raised against Galectin-3
anti- Galectin-7 antibody
FNab03318 100µg
EUR 606.3
  • Recommended dilution: WB: 1:1000-1:4000
  • IHC: 1:20-1:200
  • Immunogen: lectin, galactoside-binding, soluble, 7B
  • Uniprot ID: P47929
  • Gene ID: 653499
  • Research Area: Immunology, Cancer, Immunology, Neuroscience, Cell Division and Proliferation
Description: Antibody raised against Galectin-7
ELISA kit for Human Galectin-1
EK5323 96 tests
EUR 663.6
Description: Enzyme-linked immunosorbent assay kit for quantification of Human Galectin-1 in samples from serum, plasma, tissue homogenates and other biological fluids.
Galectin-1 Antibody
3596-100 each
EUR 379.2
Galectin-1 Antibody
3596-30T each
EUR 175.2
Galectin-1 Antibody
R34524-100UG 100 ug
EUR 339.15
Description: Additional name(s) for this target protein: Lectin, galactose binding, soluble 1; Lgals1
Galectin-1 Antibody
R34565-100UG 100 ug
EUR 339.15
Description: Additional name(s) for this target protein: Lgals1
Human Galectin 8 Antibody
33159-05111 150 ug
EUR 313.2
Human Galectin 2 Antibody
33165-05111 150 ug
EUR 313.2
Human Galectin 13 Antibody
33175-05111 150 ug
EUR 313.2
Human Galectin 10 Antibody
32652-05111 150 ug
EUR 313.2
Recombinant Human Galectin-1 Protein
PROTP09382-1 50ug
EUR 380.4
Description: Lectins, of either plant or animal origin, are carbohydrate binding proteins that interact with glycoprotein and glycolipids on the surface of animal cells. The Galectins are lectins that recognize and interact with β-galactoside moieties. Galectin-1 is an animal lectin that has been shown to interact with CD3, CD4, and CD45. It induces apoptosis of activated T-cells and T-leukemia cell lines and inhibits the protein phosphatase activity of CD45. Recombinant human Galectin-1 is a 14.5 kDa protein containing 134 amino acid residues.
Recombinant Human Galectin-1 Protein
RP00007 20 μg
EUR 208.8
Human Galectin 1 (GAL1) CLIA Kit
20-abx190336
  • EUR 9493.2
  • EUR 5058
  • EUR 1167.6
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests
  • Shipped within 5-7 working days.
Human Galectin 1 (GAL1) CLIA Kit
abx196676-96tests 96 tests
EUR 990
  • Shipped within 5-12 working days.
Human Galectin 1 (GAL1)CLIA Kit
SCA321Hu-10x96wellstestplate 10x96-wells test plate
EUR 6777.36
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Galectin 1 (GAL1) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Galectin 1 (GAL1) in serum, plasma and other biological fluids.
Human Galectin 1 (GAL1)CLIA Kit
SCA321Hu-1x48wellstestplate 1x48-wells test plate
EUR 663.31
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Galectin 1 (GAL1) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Galectin 1 (GAL1) in serum, plasma and other biological fluids.
Human Galectin 1 (GAL1)CLIA Kit
SCA321Hu-1x96wellstestplate 1x96-wells test plate
EUR 896.16
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Galectin 1 (GAL1) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Galectin 1 (GAL1) in serum, plasma and other biological fluids.
Human Galectin 1 (GAL1)CLIA Kit
SCA321Hu-5x96wellstestplate 5x96-wells test plate
EUR 3672.72
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Galectin 1 (GAL1) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Galectin 1 (GAL1) in serum, plasma and other biological fluids.
Human Galectin 1 (GAL1) CLIA Kit
4-SCA321Hu
  • EUR 6837.6
  • EUR 3673.2
  • EUR 896.4
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
  • Known also as Galectin 1 Clia kit. Alternative names of the recognized antigen: LGALS1
  • GBP
  • HLBP14
  • Galaptin
  • Lectin, Galactoside-Binding Soluble 1
  • 14 kDa laminin-binding protein
  • Lactose-binding lectin 1
  • Putative MAPK-activating protein PM12
  • S-L
  • Show more
Description: Double-antibody Sandwich chemiluminescent immunoassay for detection of Human Galectin 1 (GAL1)Serum, plasma and other biological fluids
Human Galectin-1(LGALS1) ELISA kit
CSB-EL012882HU-24T 1 plate of 24 wells
EUR 198
  • Sample volume: 50-100ul
  • Detection wavelength: 450nm
  • Assay performance time: 1 to 4 hours.
Description: Quantitativesandwich ELISA kit for measuring Human Galectin-1 (LGALS1) in samples from serum, plasma, tissue homogenates, cell lysates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Human Galectin-1(LGALS1) ELISA kit
1-CSB-EL012882HU
  • EUR 964.8
  • EUR 6118.8
  • EUR 3244.8
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
  • Sample volume: 50-100ul
  • Detection wavelength: 450nm
  • Assay performance time: 1 to 4 hours.
Description: Quantitativesandwich ELISA kit for measuring Human Galectin-1(LGALS1) in samples from serum, plasma, tissue homogenates, cell lysates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Human Galectin-1(LGALS1) ELISA kit
E01G0448-192T 192 tests
EUR 1524
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Human Galectin-1(LGALS1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human Galectin-1(LGALS1) ELISA kit
E01G0448-48 1 plate of 48 wells
EUR 624
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Human Galectin-1(LGALS1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human Galectin-1(LGALS1) ELISA kit
E01G0448-96 1 plate of 96 wells
EUR 822
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Human Galectin-1(LGALS1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human Galectin-1 (Gal-1) Antibody (Biotin Conjugate)
30005-05121 150 ug
EUR 442.8
Human Galectin 1 (GAL1) ELISA kit
201-12-3227 96 tests
EUR 528
  • This Galectin 1 ELISA kit is validated to work with samples from whole blood, serum, plasma and cell culture supernatant.
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.
Human Galectin 1 (GAL1) ELISA Kit
DLR-GAL1-Hu-48T 48T
EUR 574.8
  • Should the Human Galectin 1 (GAL1) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Human Galectin 1 (GAL1) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Human Galectin 1 (GAL1) ELISA Kit
DLR-GAL1-Hu-96T 96T
EUR 745.2
  • Should the Human Galectin 1 (GAL1) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Human Galectin 1 (GAL1) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Human Galectin 1 ELISA Kit (GAL1)
RK01436 96 Tests
EUR 625.2
Human Galectin 1 (GAL1) ELISA Kit
RDR-GAL1-Hu-48Tests 48 Tests
EUR 600
Human Galectin 1 (GAL1) ELISA Kit
RDR-GAL1-Hu-96Tests 96 Tests
EUR 830.4
Human Galectin 1(GAL1)ELISA Kit
QY-E04196 96T
EUR 433.2
Human Galectin 1 (GAL1) ELISA Kit
RD-GAL1-Hu-48Tests 48 Tests
EUR 573.6
Human Galectin 1 (GAL1) ELISA Kit
RD-GAL1-Hu-96Tests 96 Tests
EUR 794.4
Human Galectin 1 (GAL1) ELISA Kit
SEA321Hu-10x96wellstestplate 10x96-wells test plate
EUR 4091.29
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Galectin 1 (GAL1) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Galectin 1 (GAL1) in serum, plasma, tissue homogenates and other biological fluids.
Human Galectin 1 (GAL1) ELISA Kit
SEA321Hu-1x48wellstestplate 1x48-wells test plate
EUR 442.1
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Galectin 1 (GAL1) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Galectin 1 (GAL1) in serum, plasma, tissue homogenates and other biological fluids.
Human Galectin 1 (GAL1) ELISA Kit
SEA321Hu-1x96wellstestplate 1x96-wells test plate
EUR 580.15
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Galectin 1 (GAL1) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Galectin 1 (GAL1) in serum, plasma, tissue homogenates and other biological fluids.
Human Galectin 1 (GAL1) ELISA Kit
SEA321Hu-5x96wellstestplate 5x96-wells test plate
EUR 2250.68
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Galectin 1 (GAL1) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Galectin 1 (GAL1) in serum, plasma, tissue homogenates and other biological fluids.
Human Galectin 1 (GAL1) ELISA Kit
4-SEA321Hu
  • EUR 4152
  • EUR 2191.2
  • EUR 580.8
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
  • Known also as Galectin 1 elisa. Alternative names of the recognized antigen: LGALS1
  • GBP
  • HLBP14
  • Galaptin
  • Lectin, Galactoside-Binding Soluble 1
  • 14 kDa laminin-binding protein
  • Lactose-binding lectin 1
  • Putative MAPK-activating protein PM12
  • S-Lac
  • Show more
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Galectin 1 (GAL1) in samples from serum, plasma, tissue homogenates and other biological fluids with no significant corss-reactivity with analogues from other species.
Human Galectin 1 (GAL1) ELISA kit
YLA3564HU-48T 48T
EUR 435
Human Galectin 1 (GAL1) ELISA kit
YLA3564HU-96T 96T
EUR 562.5
anti- Galectin 2 antibody
FNab03310 100µg
EUR 702
  • Immunogen: lectin, galactoside-binding, soluble, 2
  • Uniprot ID: P05162
  • Research Area: Immunology, Signal Transduction, Cell Division and Proliferation
Description: Antibody raised against Galectin 2
anti- Galectin 4 antibody
FNab03311 100µg
EUR 606.3
  • Recommended dilution: WB: 1:500-1:1000
  • IHC: 1:20-1:200
  • IF: 1:10-1:100
  • Immunogen: lectin, galactoside-binding, soluble, 4
  • Uniprot ID: P56470
  • Research Area: Immunology, Signal Transduction, Cell Division and Proliferation
Description: Antibody raised against Galectin 4
anti- Galectin 8 antibody
FNab03312 100µg
EUR 606.3
  • Immunogen: lectin, galactoside-binding, soluble, 8
  • Uniprot ID: O00214
  • Research Area: Immunology, Signal Transduction, Cell Division and Proliferation
Description: Antibody raised against Galectin 8
anti- Galectin 9 antibody
FNab03313 100µg
EUR 606.3
  • Immunogen: lectin, galactoside-binding, soluble, 9
  • Uniprot ID: O00182
  • Research Area: Immunology, Signal Transduction, Cell Division and Proliferation
Description: Antibody raised against Galectin 9
Galectin 1 (GAL1) Polyclonal Antibody (Human)
4-PAA321Hu01
  • EUR 278.4
  • EUR 2742
  • EUR 688.8
  • EUR 346.8
  • EUR 249.6
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: Met1~Asp135
  • Buffer composition: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Description: A Rabbit polyclonal antibody against Human Galectin 1 (GAL1)
Galectin 1 antibody
70R-GR002 100 ug
EUR 571.2
Description: Affinity purified Rabbit polyclonal Galectin 1 antibody
Galectin 1 antibody
70R-11939 100 ug
EUR 483.6
Description: Rabbit polyclonal Galectin 1 antibody

Phage Displayed Domain Antibodies (dAb) for Detection of Allergenic Pistachio Proteins in Foods

Pistachio nuts (Pistacia vera) have been consumed by past and present-day civilizations because of their organoleptic characteristics and potential health benefits. However, they can also produce moderate to severe IgE-mediated reactions in allergic individuals. In this work, we report the isolation of the first recombinant antibodies against pistachio nut, produced without animal immunization, to be used in immunoassays for detection of allergenic pistachio in food products.
Several phage display biopanning strategies were evaluated to screen the human-based domain antibody library (dAb) in search for pistachio-specific probes. The clone producing the PVF4 phage-dAb was finally selected, and it does not cross-react with cashew despite the phylogenetic proximity with pistachio.
Western blot and matrix-assisted laser desorption/ionization tandem mass spectrometry (MALDI-TOF/TOF) analysis demonstrated that this clone recognised a unique band of ∼22 kDa related to the basic subunit of pistachio 11S globulin (allergen Pis v 2).
The PVF4 phage-dAb allowed detection of pistachio in a food matrix with a limit of detection (LOD) of 3983 mg kg-1 in an indirect phage-enzyme-linked immunosorbent assay (ELISA). The ELISA method developed was used to assess applicability of the PVF4 phage-dAb for analysis of 77 commercial food products.

Human CD163 antigen(CD163)ISA kit
QY-E05066 96T
EUR 433.2

human CD163 antigen,CD163 LISA kit
201-12-0247 96 tests
EUR 528
  • This CD163 antigen ELISA kit is validated to work with samples from whole blood, serum, plasma and cell culture supernatant.
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

human CD163 antigen,CD163 ELISA kit
YLA0098HU-48T 48T
EUR 435

human CD163 antigen,CD163 ELISA kit
YLA0098HU-96T 96T
EUR 562.5

Mouse Anti Human Cd163 Monoclonal Antibody
CABT-47162MH 25 µg
EUR 446.4

Mouse Anti Human Cd163 Monoclonal Antibody
CABT-47163MH 0.2 mg
EUR 1057.2

anti- CD163/M130 Antibody
FNab09771 100µg
EUR 658.5
  • Recommended dilution: WB: 1:500-1:1000
  • IHC: 1:300-1:1000
  • Immunogen: CD163
  • Uniprot ID: Q86VB7
  • Research Area: Signal Transduction, Metabolism, Cardiovascular, Immunology, Neuroscience, Stem Cells
Description: Antibody raised against CD163/M130 

Anti-Hu CD163 PE
1P-645-T025 25 tests
EUR 168

Anti-Hu CD163 PE
1P-645-T100 100 tests
EUR 288

Anti-Hu CD163 APC
1A-645-T025 25 tests
EUR 168

Anti-Hu CD163 APC
1A-645-T100 100 tests
EUR 288

Anti-Hu CD163 Purified
11-645-C025 0.025 mg
EUR 118.8

Anti-Hu CD163 Purified
11-645-C100 0.1 mg
EUR 189.6

Human CD163 ELISA Kit
LF-EK50890 1×96T
EUR 777.6

Human CD163 ELISA Kit
ELA-E1726h 96 Tests
EUR 988.8

Human CD163 shRNA Plasmid
20-abx956176
  • EUR 961.2
  • EUR 1345.2
  • 150 µg
  • 300 µg
  • Shipped within 15-20 working days.

Anti-Hu CD163 PE-Cy7
T7-645-T025 25 tests
EUR 184.8

Anti-Hu CD163 PE-Cy7
T7-645-T100 100 tests
EUR 321.6

Anti-Hu CD163 PerCP-Cy5.5
T9-645-T025 25 tests
EUR 235.2

Anti-Hu CD163 PerCP-Cy5.5
T9-645-T100 100 tests
EUR 422.4

PE Anti-human CD163 Antibody *GHI/61*
116301K0 25 tests
EUR 383
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

PE Anti-human CD163 Antibody *GHI/61*
116301K1 100 tests
EUR 995
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

PE Anti-human CD163 Antibody *GHI/61*
116301K2 500 tests
EUR 2984
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

APC Anti-human CD163 Antibody *GHI/61*
116301B0 25 tests
EUR 383
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

APC Anti-human CD163 Antibody *GHI/61*
116301B1 100 tests
EUR 995
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

APC Anti-human CD163 Antibody *GHI/61*
116301B2 500 tests
EUR 2984
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

FITC Anti-human CD163 Antibody *GHI/61*
116301G0 100 tests
EUR 547
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

FITC Anti-human CD163 Antibody *GHI/61*
116301G1 500 tests
EUR 2051
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

TRITC Anti-human CD163 Antibody *GHI/61*
116301H0 100 tests
EUR 547
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

TRITC Anti-human CD163 Antibody *GHI/61*
116301H1 500 tests
EUR 2051
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

PerCP Anti-human CD163 Antibody *GHI/61*
116301S0 25 tests
EUR 383
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

PerCP Anti-human CD163 Antibody *GHI/61*
116301S1 100 tests
EUR 995
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

PerCP Anti-human CD163 Antibody *GHI/61*
116301S2 500 tests
EUR 2984
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

PacBlue Anti-human CD163 Antibody *GHI/61*
116301I0 100 tests
EUR 547
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

PacBlue Anti-human CD163 Antibody *GHI/61*
116301I1 500 tests
EUR 2051
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

Purified Anti-human CD163 Antibody *GHI/61*
11630000 100 ug
EUR 365
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

PacOrange Anti-human CD163 Antibody *GHI/61*
116301J0 100 tests
EUR 547
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

PacOrange Anti-human CD163 Antibody *GHI/61*
116301J1 500 tests
EUR 2051
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

Canine CD163 antigen(CD163 LISA kit
GA-E0062CN-48T 48T
EUR 482.4

Canine CD163 antigen(CD163 LISA kit
GA-E0062CN-96T 96T
EUR 820.8

Human CellExp? CD163, Human recombinant
9226-10 each
EUR 366

Human CellExp? CD163, Human recombinant
9226-50 each
EUR 927.6

Porcine CD163 antigen,CD163 LISA kit
GA-E0045PC-48T 48T
EUR 436.8

Porcine CD163 antigen,CD163 LISA kit
GA-E0045PC-96T 96T
EUR 708

Porcine CD163 antigen,CD163 LISA kit
YLA0139PO-48T 48T
EUR 502.5

Porcine CD163 antigen,CD163 LISA kit
YLA0139PO-96T 96T
EUR 618.75

ELISA kit for Human CD163
EK5296 96 tests
EUR 663.6
Description: Enzyme-linked immunosorbent assay kit for quantification of Human CD163 in samples from serum, plasma, tissue homogenates and other biological fluids.

CD163 Antibody
49553-100ul 100ul
EUR 399.6

CD163 Antibody
49553-50ul 50ul
EUR 286.8

CD163 antibody
10R-CD163aRT 250 ug
EUR 859.2
Description: Mouse monoclonal CD163 antibody

CD163 antibody
10R-6496 100 ug
EUR 243.6
Description: Mouse monoclonal CD163 antibody

CD163 Antibody
ABD8235 100 ug
EUR 525.6

CD163 Antibody
37157-100ul 100ul
EUR 302.4

Cd163 Antibody
1-CSB-PA650452LA01MO
  • EUR 380.4
  • EUR 402
  • 100ug
  • 50ug
  • Form: Liquid
  • Buffer: Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against Cd163. Recognizes Cd163 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB; Recommended dilution: WB:1:500-1:2000

CD163 Antibody
20-abx242956
  • EUR 560.4
  • EUR 393.6
  • 100 ul
  • 50 ul
  • Shipped within 1-2 weeks.

CD163 Antibody
1-CSB-PA969205
  • EUR 380.4
  • EUR 292.8
  • 100ul
  • 50ul
  • Form: Liquid
  • Buffer: -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol Antigen affinity purification
Description: A polyclonal antibody against CD163. Recognizes CD163 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:1000-1:2000, IHC:1:25-1:100

CD163 Antibody
DF8235 200ul
EUR 420

CD163 Antibody
1-CSB-PA801238LA01HU
  • EUR 380.4
  • EUR 402
  • 100ul
  • 50ul
  • Form: Liquid
  • Buffer: Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against CD163. Recognizes CD163 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IF; Recommended dilution: IF:1:50-1:200

CD163 Antibody
F50316-0.08ML 0.08 ml
EUR 140.25
Description: CD163 is an acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. The protein may play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. It binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. And it exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. It also induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1.

CD163 Antibody
F50316-0.4ML 0.4 ml
EUR 322.15
Description: CD163 is an acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. The protein may play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. It binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. And it exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. It also induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1.

CD163 Antibody
RQ4259 100 ug
EUR 356.15
Description: CD163 (Cluster of Differentiation 163) is a protein that in humans is encoded by the CD163 gene. The protein encoded by this gene is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.

CD163 Antibody
R30973 100 ug
EUR 356.15
Description: CD163 (Cluster of Differentiation 163) is a human protein encoded by the CD163 gene. The receptor belongs to the scavenger receptor cysteine rich family type B and consists of an 1048 amino acid residues extracellular domain, a single transmembrane segment and a cytoplasmic tail with several splice variants. CD163 is a scavenger receptor for the hemoglobin-haptoglobincomplex. Using FISH, somatic cell hybrid analysis, and radiation hybrid analysis, Stover et al.(2000) mapped the CD163 gene to chromosome 12p13.3. Specific CD163-mediated endocytosis of haptoglobin-hemoglobin complexes was measurable in cells transfected with CD163 cDNA and in CD163-expressing myelomonocytic lymphoma cells.CD163 expression in monocytes promoted bacteria-induced proinflammatory cytokine production that could be blocked by anti-CD163 antibodies. Cells expressing human CD163 and recombinant protein containing the extracellular domain of CD163 supported adhesion of erythroblastic cells.

CD163 Antibody
R30974 100 ug
EUR 356.15
Description: Cluster of Differentiation 163, also known as Hemoglobin scavenger receptor, is a human protein encoded by the CD163 gene. The receptor belongs to the scavenger receptor cysteine rich family type B and consists of an 1048 amino acid residues extracellular domain, a single transmembrane segment and a cytoplasmic tail with several splice variants. CD163 is a scavenger receptorfor the hemoglobin-haptoglobin complex. Using FISH, somatic cell hybrid analysis, and radiation hybrid analysis, Stover et al.(2000) mapped the gene to chromosome 12p13.3. CD163 expression in monocytes promoted bacteria-induced proinflammatory cytokine production that could be blocked by CD163 antibody. Cells expressing human CD163 and recombinant protein containing the extracellular domain of CD163 supported adhesion of erythroblastic cells.

CD163 Antibody
V8227-100UG 100 ug
EUR 349.3
Description: This MAb recognizes a protein of 140kDa, identified as CD163. It has been identified as an acute phase-regulated transmembrane protein whose function is to mediate the endocytosis of haptoglobin-hemoglobin complexes. This receptor is expressed on the surface of monocytes with low expression and on tissue macrophages, histiocytes with high expression. Staining with anti-CD163 has been helpful to distinguish synovial macrophages from synovial intimal fibroblasts in rheumatoid arthritis, where its specificity for macrophages was found to be superior to that of anti-CD68. Increased levels of CD163 were also detected in patients with microbial infections and myelomonocytic leukemias. Anti-CD163 is of considerable value for selective identification of monocytes and macrophages at a certain stage of differentiation and is suitable for diagnosing myelomonocytic or monocytic leukaemia and neoplasms of true histiocytic origin. CD163 is positive in skin (histiocytes), gut, Kupffer cells, a few alveolar macrophages, macrophages in the placenta, and in macrophages in inflamed tissues including tumor tissue.

CD163 Antibody
V8227-20UG 20 ug
EUR 153.3
Description: This MAb recognizes a protein of 140kDa, identified as CD163. It has been identified as an acute phase-regulated transmembrane protein whose function is to mediate the endocytosis of haptoglobin-hemoglobin complexes. This receptor is expressed on the surface of monocytes with low expression and on tissue macrophages, histiocytes with high expression. Staining with anti-CD163 has been helpful to distinguish synovial macrophages from synovial intimal fibroblasts in rheumatoid arthritis, where its specificity for macrophages was found to be superior to that of anti-CD68. Increased levels of CD163 were also detected in patients with microbial infections and myelomonocytic leukemias. Anti-CD163 is of considerable value for selective identification of monocytes and macrophages at a certain stage of differentiation and is suitable for diagnosing myelomonocytic or monocytic leukaemia and neoplasms of true histiocytic origin. CD163 is positive in skin (histiocytes), gut, Kupffer cells, a few alveolar macrophages, macrophages in the placenta, and in macrophages in inflamed tissues including tumor tissue.

CD163 Antibody
V8227SAF-100UG 100 ug
EUR 349.3
Description: This MAb recognizes a protein of 140kDa, identified as CD163. It has been identified as an acute phase-regulated transmembrane protein whose function is to mediate the endocytosis of haptoglobin-hemoglobin complexes. This receptor is expressed on the surface of monocytes with low expression and on tissue macrophages, histiocytes with high expression. Staining with anti-CD163 has been helpful to distinguish synovial macrophages from synovial intimal fibroblasts in rheumatoid arthritis, where its specificity for macrophages was found to be superior to that of anti-CD68. Increased levels of CD163 were also detected in patients with microbial infections and myelomonocytic leukemias. Anti-CD163 is of considerable value for selective identification of monocytes and macrophages at a certain stage of differentiation and is suitable for diagnosing myelomonocytic or monocytic leukaemia and neoplasms of true histiocytic origin. CD163 is positive in skin (histiocytes), gut, Kupffer cells, a few alveolar macrophages, macrophages in the placenta, and in macrophages in inflamed tissues including tumor tissue.

CD163 Antibody
V8415-100UG 100 ug
EUR 349.3
Description: This MAb recognizes a protein of 140kDa, identified as CD163. It has been identified as an acute phase-regulated transmembrane protein whose function is to mediate the endocytosis of haptoglobin-hemoglobin complexes. This receptor is expressed on the surface of monocytes with low expression and on tissue macrophages, histiocytes with high expression. Staining with anti-CD163 has been helpful to distinguish synovial macrophages from synovial intimal fibroblasts in rheumatoid arthritis, where its specificity for macrophages was found to be superior to that of anti-CD68. Increased levels of CD163 were also detected in patients with microbial infections and myelomonocytic leukemias. Anti-CD163 is of considerable value for selective identification of monocytes and macrophages at a certain stage of differentiation and is suitable for diagnosing myelomonocytic or monocytic leukaemia and neoplasms of true histiocytic origin. CD163 is positive in skin (histiocytes), gut, Kupffer cells, a few alveolar macrophages, macrophages in the placenta, and in macrophages in inflamed tissues including tumor tissue.

CD163 Antibody
V8415-20UG 20 ug
EUR 153.3
Description: This MAb recognizes a protein of 140kDa, identified as CD163. It has been identified as an acute phase-regulated transmembrane protein whose function is to mediate the endocytosis of haptoglobin-hemoglobin complexes. This receptor is expressed on the surface of monocytes with low expression and on tissue macrophages, histiocytes with high expression. Staining with anti-CD163 has been helpful to distinguish synovial macrophages from synovial intimal fibroblasts in rheumatoid arthritis, where its specificity for macrophages was found to be superior to that of anti-CD68. Increased levels of CD163 were also detected in patients with microbial infections and myelomonocytic leukemias. Anti-CD163 is of considerable value for selective identification of monocytes and macrophages at a certain stage of differentiation and is suitable for diagnosing myelomonocytic or monocytic leukaemia and neoplasms of true histiocytic origin. CD163 is positive in skin (histiocytes), gut, Kupffer cells, a few alveolar macrophages, macrophages in the placenta, and in macrophages in inflamed tissues including tumor tissue.

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