The Role of IgG Subclass in Antibody-Mediated
The Role of IgG Subclass in Antibody-Mediated Protection against Carbapenem-Resistant Klebsiella pneumoniae
- Monoclonal antibodies (MAbs) have the potential to assist in the battle against multidrug-resistant bacteria such as carbapenem-resistant Klebsiella pneumoniae (CR-Kp).
- However, the characteristics by which these antibodies (Abs) function, such as the role of antibody subclass, must be determined before such modalities can be carried from the bench to the bedside. We performed a subclass switch on anticapsular monoclonal murine IgG3 (mIgG3) hybridomas and identified and purified a murine IgG1 (mIgG1) hybridoma line through sib selection. We then compared the ability of the mIgG1 and mIgG3 antibodies to control CR-Kp sequence type 258 (ST258) infection both in vitro and in vivo.
- We found by enzyme-limited immunosorbent assay (ELISA) and flow cytometry that mIgG3 has superior binding to the CR-Kp capsular polysaccharide (CPS) and superior agglutinating ability compared to mIgG1 The mIgG3 also, predictably, had better complement-mediated serum bactericidal activity than the mIgG1 and also promoted neutrophil-mediated killing at concentrations lower than that of the mIgG1 In contrast, the mIgG1 had marginally better activity in improving macrophage-mediated phagocytosis.
- Comparing their activities in a pulmonary infection model with wild-type as well as neutropenic mice, both antibodies reduced organ burden in a nonlethal challenge, regardless of neutrophil status, with mIgG1 having the highest overall burden reduction in both scenarios.
- However, at a lethal inoculum, both antibodies showed reduced efficacy in neutropenic mice, with mIgG3 retaining the most activity. These findings suggest the viability of monoclonal Ab adjunctive therapy in neutropenic patients that cannot mount their own immune response, while also providing some insight into the relative contributions of immune mediators in CR-Kp protection.IMPORTANCE Carbapenem-resistant Klebsiella pneumoniae is an urgent public health threat that causes life-threatening infections in immunocompromised hosts.
- Its resistance to nearly all antibiotics necessitates novel strategies to treat it, including the use of monoclonal antibodies. Monoclonal antibodies are emerging as important adjuncts to traditional pharmaceuticals, and studying how they protect against specific bacteria such as Klebsiella pneumoniae is crucial to their development as effective therapies.
- Antibody subclass is often overlooked but is a major factor in how an antibody interacts with other mediators of immunity. This paper is the first to examine how the subclass of anticapsular monoclonal antibodies can affect efficacy against CR-Kp Additionally, this work sheds light on the viability of monoclonal antibody therapy in neutropenic patients, who are most vulnerable to CR-Kp infection.
enviroshieldproducts
 Human Galectin-1 |
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| MBS691972-001mg | MyBiosource | 0.01mg | EUR 300 |
Human Galectin-1 |
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| MBS691972-005mg | MyBiosource | 0.05mg | EUR 450 |
Human Galectin-1 |
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| MBS691972-5x005mg | MyBiosource | 5x0.05mg | EUR 1725 |
 Galectin-1 Human |
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| GWB-DAB7E1 | GenWay Biotech | 0.05 mg | Ask for price |
 Galectin-1， Human |
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| E34M148H | EnoGene | 5 μg | EUR 155 |
 Galectin-1, Human |
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| MBS8575305-0005mg | MyBiosource | 0.005mg | EUR 245 |
Galectin-1, Human |
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| MBS8575305-5x0005mg | MyBiosource | 5x0.005mg | EUR 940 |
 Mouse anti Human Galectin-1 Antibody, Mouse MAb |
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| MBS2543309-006mL | MyBiosource | 0.06mL | EUR 250 |
Mouse anti Human Galectin-1 Antibody, Mouse MAb |
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| MBS2543309-012mL | MyBiosource | 0.12mL | EUR 370 |
Mouse anti Human Galectin-1 Antibody, Mouse MAb |
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| MBS2543309-02mL | MyBiosource | 0.2mL | EUR 550 |
 Elisa Kit) Human Galectin( Galectin) Elisa Kit |
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| EK711568 | AFG Bioscience LLC | 96 Wells | EUR 0.18 |
 Antibody) Human Galectin-1 (Gal-1) Antibody |
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| 30005-05111 | AssayPro | 150 ug | EUR 215 |
 RABBIT ANTI HUMAN GALECTIN-3:Biotin |
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| MBS222930-005mg | MyBiosource | 0.05mg | EUR 655 |
RABBIT ANTI HUMAN GALECTIN-3:Biotin |
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| MBS222930-5x005mg | MyBiosource | 5x0.05mg | EUR 2780 |
 Rabbit Anti-Human Galectin-1 |
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| 102-PA131 | Angio Proteomie | 100ug | EUR 240 |
 Anti-Human Galectin-1 Antibody |
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| 102-P131 | ReliaTech | 100 µg | EUR 245.7 |
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Description: Galectin-1, also known as L14, BHL and galaptin, is a monomeric or homodimeric prototype galectin that is expressed in a variety of cells and tissues including muscle, heart, liver, prostate, lymph nodes, spleen, thymus, placenta, testis, retina, macrophages, B cells, T cells, dendritic cells, and tumor cells. It preferentially binds laminin, fibronectin, 90K/Mac2BP, CD45, CD43, CD7, CD2, CD3, and ganglioside GM1. Galectin-1 modulates cell growth and proliferation, either positively or negatively, depending on the cell type and activation status. It controls cell survival by inducing apoptosis of activated T cells and immature thymocytes. It modulates cytokine secretion by inducing Th2 type cytokines and inhibiting proinflammatory cytokine production. Galectin1 can also modulate cel-lcell as well as cell-lmatrix interactions and depending on the cell type and developmental stage, promote cell attachment or detachment. Galectin-1 has immunosuppressive and anti-inflammatory properties and has been shown to suppress acute and chronic inflammation and autoimmunity. Human and mouse galectin1 share about 88% amino acid sequence similarity. |
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Anti-Human Galectin-1 Antibody |
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| 102-PA131AG | ReliaTech | 50 µg | EUR 157.5 |
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Description: Galectin-1, also known as L14, BHL and galaptin, is a monomeric or homodimeric prototype galectin that is expressed in a variety of cells and tissues including muscle, heart, liver, prostate, lymph nodes, spleen, thymus, placenta, testis, retina, macrophages, B cells, T cells, dendritic cells, and tumor cells. It preferentially binds laminin, fibronectin, 90K/Mac2BP, CD45, CD43, CD7, CD2, CD3, and ganglioside GM1. Galectin-1 modulates cell growth and proliferation, either positively or negatively, depending on the cell type and activation status. It controls cell survival by inducing apoptosis of activated T cells and immature thymocytes. It modulates cytokine secretion by inducing Th2 type cytokines and inhibiting proinflammatory cytokine production. Galectin1 can also modulate cel-lcell as well as cell-lmatrix interactions and depending on the cell type and developmental stage, promote cell attachment or detachment. Galectin-1 has immunosuppressive and antiinflammatory properties and has been shown to suppress acute and chronic inflammation and autoimmunity. Human and mouse galectin1 share about 88% amino acid sequence similarity. |
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Anti-Human Galectin-1 Antibody |
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| 102-PA131S | ReliaTech | 100 µg | EUR 126 |
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Description: Galectin-1, also known as L14, BHL and galaptin, is a monomeric or homodimeric prototype galectin that is expressed in a variety of cells and tissues including muscle, heart, liver, prostate, lymph nodes, spleen, thymus, placenta, testis, retina, macrophages, B cells, T cells, dendritic cells, and tumor cells. It preferentially binds laminin, fibronectin, 90K/Mac2BP, CD45, CD43, CD7, CD2, CD3, and ganglioside GM1. Galectin-1 modulates cell growth and proliferation, either positively or negatively, depending on the cell type and activation status. It controls cell survival by inducing apoptosis of activated T cells and immature thymocytes. It modulates cytokine secretion by inducing Th2 type cytokines and inhibiting proinflammatory cytokine production. Galectin1 can also modulate cel-lcell as well as cell-lmatrix interactions and depending on the cell type and developmental stage, promote cell attachment or detachment. Galectin-1 has immunosuppressive and antiinflammatory properties and has been shown to suppress acute and chronic inflammation and autoimmunity. Human and mouse galectin1 share about 88% amino acid sequence similarity. |
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) Human Galectin-1 (LGALS1) |
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| 1-CSB-EP012882HU | Cusabio |
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Description: Recombinant Human Galectin-1(LGALS1) expressed in E.coli |
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Human Galectin-1 (LGALS1) |
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| 1-CSB-EP012882HUb1 | Cusabio |
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Description: Recombinant Human Galectin-1(LGALS1) expressed in E.coli |
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) human anti-human galectin-3 mAb(2D) |
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| E4A09H10-2D | EnoGene | 50ug | EUR 275 |
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Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody |
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) human anti-human galectin-3 mAb(5F) |
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| E4A09H10-5F | EnoGene | 50ug | EUR 275 |
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Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody |
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) human anti-human galectin-3 mAb(A5) |
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| E4A09H10-A5 | EnoGene | 50ug | EUR 275 |
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Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody |
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) human anti-human galectin-3 mAb(G2) |
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| E4A09H10-G2 | EnoGene | 50ug | EUR 275 |
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Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody |
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) human anti-human galectin-3 mAb(H3) |
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| E4A09H10-H3 | EnoGene | 50ug | EUR 275 |
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Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody |
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) human anti-human galectin-3 mAb(T1) |
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| E4A09H10-T1 | EnoGene | 50ug | EUR 275 |
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Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody |
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 Human Galectin-1 Protein |
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| PRP1033-100g | Abbkine | 100 µg | EUR 309 |
Human Galectin-1 Protein |
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| PRP1033-1mg | Abbkine | 1 mg | EUR 1099 |
Human Galectin-1 Protein |
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| PRP1033-20g | Abbkine | 20 µg | EUR 79 |
) human anti-human galectin-3 mAb(N13) |
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| E4A09H10-N13 | EnoGene | 50ug | EUR 275 |
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Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody |
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 Mouse anti Human Galectin-1/LGALS1 Monoclonal Antibody |
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| MBS2543310-006mL | MyBiosource | 0.06mL | EUR 250 |
Mouse anti Human Galectin-1/LGALS1 Monoclonal Antibody |
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| MBS2543310-012mL | MyBiosource | 0.12mL | EUR 370 |
Mouse anti Human Galectin-1/LGALS1 Monoclonal Antibody |
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| MBS2543310-02mL | MyBiosource | 0.2mL | EUR 550 |
 Anti Human Galectin-3 Polyclonal Antibody |
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| KH040 | Sceti | each | EUR 778.8 |
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Description: The Anti Human Galectin-3 Polyclonal Antibody is available in Europe and for worldwide shipping via Gentaur. |
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 Rabbit anti Human Galectin-1/LGALS1 Monoclonal Antibody |
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| MBS2543912-006mL | MyBiosource | 0.06mL | EUR 250 |
Rabbit anti Human Galectin-1/LGALS1 Monoclonal Antibody |
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| MBS2543912-012mL | MyBiosource | 0.12mL | EUR 370 |
Rabbit anti Human Galectin-1/LGALS1 Monoclonal Antibody |
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| MBS2543912-02mL | MyBiosource | 0.2mL | EUR 550 |
Rabbit anti Human Galectin-1/LGALS1 Monoclonal Antibody |
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| MBS2543913-006mL | MyBiosource | 0.06mL | EUR 250 |
Rabbit anti Human Galectin-1/LGALS1 Monoclonal Antibody |
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| MBS2543913-012mL | MyBiosource | 0.12mL | EUR 370 |
Rabbit anti Human Galectin-1/LGALS1 Monoclonal Antibody |
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| MBS2543913-02mL | MyBiosource | 0.2mL | EUR 550 |
ELISA Kit) Human Galectin-3(Galectin-3)ELISA Kit |
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| YLA0216HU-48T | Shanghai YL Biotech | 48T | Ask for price |
Human Galectin-3(Galectin-3)ELISA Kit |
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| YLA0216HU-96T | Shanghai YL Biotech | 96T | Ask for price |
 Recombinant Human Galectin-1 |
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| 7-00427 | CHI Scientific | 10µg | Ask for price |
Recombinant Human Galectin-1 |
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| 7-00428 | CHI Scientific | 50µg | Ask for price |
Recombinant Human Galectin-1 |
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| 7-00429 | CHI Scientific | 1mg | Ask for price |
Recombinant Human Galectin-1 |
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| AP60482 | SAB | 100ug | EUR 449 |
Recombinant Human Galectin-1 |
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| cyt-544-10g | ProSpec Tany | 10µg | EUR 60 |
Recombinant Human Galectin-1 |
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| cyt-544-1mg | ProSpec Tany | 1mg | EUR 1350 |
Recombinant Human Galectin-1 |
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| cyt-544-50g | ProSpec Tany | 50µg | EUR 145 |
Recombinant Human Galectin-1 |
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| HEOPP-0710 | Cyagen | 10ug | Ask for price |
 RECOMBINANT HUMAN GALECTIN-1 |
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| GWB-BEA2B0 | GenWay Biotech | 0.05 mg | Ask for price |
RECOMBINANT HUMAN GALECTIN-1 |
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| MBS232407-005mg | MyBiosource | 0.05mg | EUR 500 |
RECOMBINANT HUMAN GALECTIN-1 |
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| MBS232407-5x005mg | MyBiosource | 5x0.05mg | EUR 2070 |
Recombinant Human Galectin-1 |
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| MBS143303-001mg | MyBiosource | 0.01mg | EUR 240 |
Recombinant Human Galectin-1 |
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| MBS143303-005mg | MyBiosource | 0.05mg | EUR 310 |
Recombinant Human Galectin-1 |
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| MBS143303-1mg | MyBiosource | 1mg | EUR 1515 |
Recombinant Human Galectin-1 |
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| MBS143303-5x1mg | MyBiosource | 5x1mg | EUR 6485 |
Recombinant Human Galectin-1 |
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| MBS966052-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 255 |
Recombinant Human Galectin-1 |
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| MBS966052-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 405 |
Recombinant Human Galectin-1 |
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| MBS966052-1mgEColi | MyBiosource | 1mg(E-Coli) | EUR 1425 |
Recombinant Human Galectin-1 |
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| MBS966052-5x1mgEColi | MyBiosource | 5x1mg(E-Coli) | EUR 6215 |
Recombinant Human Galectin-1 |
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| MBS9420409-002mg | MyBiosource | 0.02mg | EUR 285 |
Recombinant Human Galectin-1 |
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| MBS9420409-01mg | MyBiosource | 0.1mg | EUR 470 |
Recombinant Human Galectin-1 |
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| MBS9420409-1mg | MyBiosource | 1mg | EUR 1785 |
Recombinant Human Galectin-1 |
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| MBS9420409-5x1mg | MyBiosource | 5x1mg | EUR 7885 |
Recombinant Human Galectin-1 |
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| P1229-.01 | ApexBio | 10ug | EUR 108.8 |
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Description: Peptides & Proteins|Recombinant Proteins |
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Recombinant Human Galectin-1 |
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| P1229-.1 | ApexBio | 10X10ug | EUR 450.4 |
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Description: Peptides & Proteins|Recombinant Proteins |
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Recombinant Human Galectin-1 |
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| P1229-.5 | ApexBio | 50X10ug | EUR 1010.4 |
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Description: Peptides & Proteins|Recombinant Proteins |
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 Anti- Galectin-1 Antibody |
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| GWB-6F6392 | GenWay Biotech | 0.1 mg | Ask for price |
 anti- Galectin-1 antibody |
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| FNab03314 | FN Test | 100µg | EUR 606.3 |
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Description: Antibody raised against Galectin-1 |
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anti- Galectin-1 antibody |
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| FNab03315 | FN Test | 100µg | EUR 658.5 |
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Description: Antibody raised against Galectin-1 |
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 Human Galectin 1 Protein |
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| 20-abx066725 | Abbexa |
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 Human Galectin-1 Antibody Pair Set |
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| ABPR-ZB244 | Creative Diagnostics | 5 plates, 15 plates | Ask for price |
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Description: Quantitative determination of Human Galectin-1 |
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 Galectin-1, human recombinant |
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| 4648-10 | Biovision | each | EUR 170.4 |
Galectin-1, human recombinant |
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| 4648-1000 | Biovision | each | EUR 1977.6 |
Galectin-1, human recombinant |
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| 4648-50 | Biovision | each | EUR 314.4 |
, (recombinant)) Galectin-1 (human), (recombinant) |
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| MBS567229-01mg | MyBiosource | 0.1mg | EUR 750 |
Galectin-1 (human), (recombinant) |
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| MBS567229-5x01mg | MyBiosource | 5x0.1mg | EUR 3320 |
 Galectin-1 Human Recombinant |
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| rAP-2252 | Angio Proteomie | Inquiry | Ask for price |
 Human Galectin-1 ELISA Kit |
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| EK5323 | SAB | 96 tests | EUR 599 |
Human Galectin-1 ELISA Kit |
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| IHULGALS1KT | Innovative research | each | EUR 702 |
|
Description: Human Galectin-1 ELISA Kit |
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Human Galectin-1 ELISA Kit |
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| MBS2883188-10x96StripWells | MyBiosource | 10x96-Strip-Wells | EUR 3290 |
Human Galectin-1 ELISA Kit |
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| MBS2883188-48StripWells | MyBiosource | 48-Strip-Wells | EUR 340 |
Human Galectin-1 ELISA Kit |
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| MBS2883188-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 1800 |
Human Galectin-1 ELISA Kit |
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| MBS2883188-96StripWells | MyBiosource | 96-Strip-Wells | EUR 435 |
Human Galectin-1 ELISA Kit |
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| MBS8414441-1Kit | MyBiosource | 1Kit | EUR 755 |
Human Galectin-1 ELISA Kit |
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| MBS8414441-5x1Kit | MyBiosource | 5x1Kit | EUR 3455 |
Human Galectin-1 ELISA Kit |
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| MBS765403-10x96StripWells | MyBiosource | 10x96-Strip-Wells | EUR 3900 |
Human Galectin-1 ELISA Kit |
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| MBS765403-48StripWells | MyBiosource | 48-Strip-Wells | EUR 340 |
Human Galectin-1 ELISA Kit |
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| MBS765403-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 2045 |
Human Galectin-1 ELISA Kit |
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| MBS765403-96StripWells | MyBiosource | 96-Strip-Wells | EUR 455 |
 Anti-Human Galectin-1 Azide Free |
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| MBS335796-02mg | MyBiosource | 0.2mg | EUR 290 |
Anti-Human Galectin-1 Azide Free |
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| MBS335796-05mg | MyBiosource | 0.5mg | EUR 555 |
Anti-Human Galectin-1 Azide Free |
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| MBS335796-5x05mg | MyBiosource | 5x0.5mg | EUR 2465 |
Anti-Human Galectin-1 Azide Free |
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| MBS335797-02mg | MyBiosource | 0.2mg | EUR 290 |
Anti-Human Galectin-1 Azide Free |
|||
| MBS335797-05mg | MyBiosource | 0.5mg | EUR 555 |
Anti-Human Galectin-1 Azide Free |
|||
| MBS335797-5x05mg | MyBiosource | 5x0.5mg | EUR 2465 |
 Recombinant Human Galectin 1 |
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| MBS7609301-005mg | MyBiosource | 0.05mg | EUR 345 |
Recombinant Human Galectin 1 |
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| MBS7609301-02mg | MyBiosource | 0.2mg | EUR 635 |
Recombinant Human Galectin 1 |
|||
| MBS7609301-1mg | MyBiosource | 1mg | EUR 1800 |
Recombinant Human Galectin 1 |
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| MBS7609301-5x1mg | MyBiosource | 5x1mg | EUR 6955 |
 Protein) Human Galectin-1 (LEG1) Protein |
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| abx690252-100g | Abbexa | 100 µg | EUR 837.5 |
Human Galectin-1 (LEG1) Protein |
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| abx690252-20g | Abbexa | 20 µg | EUR 350 |
 Human Galectin 1 CLIA Kit |
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| MBS8425460-INQUIRE | MyBiosource | INQUIRE | Ask for price |
 ELISA) Human Galectin 1 (GAL1) ELISA |
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| KT-15605 | Kamiya Biomedical Company | 96 tests | EUR 952 |
 Protein) Human Galectin-1 (LGALS1) Protein |
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| abx670211-50ug | Abbexa | 50 ug | EUR 661.2 |
 Galectin-1, 1-135aa, Human, E Coli |
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| MBS203386-002mg | MyBiosource | 0.02mg | EUR 190 |
Galectin-1, 1-135aa, Human, E Coli |
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| MBS203386-01mg | MyBiosource | 0.1mg | EUR 280 |
Galectin-1, 1-135aa, Human, E Coli |
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| MBS203386-05mg | MyBiosource | 0.5mg | EUR 570 |
Galectin-1, 1-135aa, Human, E Coli |
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| MBS203386-5x05mg | MyBiosource | 5x0.5mg | EUR 2325 |
 Human Galectin 1 ELISA Kit |
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| MBS721030-48StripWellsCompetitive | MyBiosource | 48-Strip-Wells-(Competitive) | EUR 485 |
Human Galectin 1 ELISA Kit |
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| MBS721030-48StripWellsSandwich | MyBiosource | 48-Strip-Wells-(Sandwich) | EUR 485 |
Human Galectin 1 ELISA Kit |
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| MBS721030-5x96StripWellsCompetitive | MyBiosource | 5x96-Strip-Wells-(Competitive) | EUR 3020 |
Human Galectin 1 ELISA Kit |
|||
| MBS721030-96StripWellsCompetitive | MyBiosource | 96-Strip-Wells-(Competitive) | EUR 690 |
Human Galectin 1 ELISA Kit |
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| MBS721030-96StripWellsSandwich | MyBiosource | 96-Strip-Wells-(Sandwich) | EUR 690 |
 Human Galectin 1 ELISA kit |
|||
| E01A4669 | BlueGene | 96T | EUR 700 |
|
Description: ELISA |
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Human Galectin 1 ELISA kit |
|||
| E01G0038-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A sandwich ELISA for quantitative measurement of Human Galectin 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Human Galectin 1 ELISA kit |
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| E01G0038-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A sandwich ELISA for quantitative measurement of Human Galectin 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Human Galectin 1 ELISA kit |
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| E01G0038-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A sandwich ELISA for quantitative measurement of Human Galectin 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Human Galectin 1 ELISA Kit |
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| MBS029650-10x96StripWells | MyBiosource | 10x96-Strip-Wells | EUR 6725 |
Human Galectin 1 ELISA Kit |
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| MBS029650-48StripWells | MyBiosource | 48-Strip-Wells | EUR 550 |
Human Galectin 1 ELISA Kit |
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| MBS029650-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 3420 |
Human Galectin 1 ELISA Kit |
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| MBS029650-96StripWells | MyBiosource | 96-Strip-Wells | EUR 765 |
Human Galectin 1 ELISA Kit |
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| IHUGAL1KT | Innovative research | each | EUR 667 |
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Description: Human Galectin 1 ELISA Kit |
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Human Galectin 1 ELISA Kit |
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| MBS8248373-5x96Tests | MyBiosource | 5x96Tests | EUR 2880 |
Human Galectin 1 ELISA Kit |
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| MBS8248373-96Tests | MyBiosource | 96Tests | EUR 645 |
 Human Galectin-3 |
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| MBS691554-001mg | MyBiosource | 0.01mg | EUR 300 |
Human Galectin-3 |
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| MBS691554-005mg | MyBiosource | 0.05mg | EUR 450 |
Human Galectin-3 |
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| MBS691554-5x005mg | MyBiosource | 5x0.05mg | EUR 1725 |
 Mouse Monoclonal anti-human Galectin-1 |
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| hAP-1228 | Angio Proteomie | 50ug | EUR 400 |
 Sheep Polyclonal anti-Human Galectin-1 |
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| hAP-5905 | Angio Proteomie | 50ug | EUR 400 |
 ELISA Kit) Human Galectin-1 (GAL-1) ELISA Kit |
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| MBS3800380-10x96StripWells | MyBiosource | 10x96-Strip-Wells | EUR 6725 |
Human Galectin-1 (GAL-1) ELISA Kit |
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| MBS3800380-48StripWells | MyBiosource | 48-Strip-Wells | EUR 550 |
Human Galectin-1 (GAL-1) ELISA Kit |
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| MBS3800380-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 3420 |
Human Galectin-1 (GAL-1) ELISA Kit |
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| MBS3800380-96StripWells | MyBiosource | 96-Strip-Wells | EUR 765 |
Phage Displayed Domain Antibodies (dAb) for Detection of Allergenic Pistachio Proteins in Foods
Pistachio nuts (Pistacia vera) have been consumed by past and present-day civilizations because of their organoleptic characteristics and potential health benefits. However, they can also produce moderate to severe IgE-mediated reactions in allergic individuals. In this work, we report the isolation of the first recombinant antibodies against pistachio nut, produced without animal immunization, to be used in immunoassays for detection of allergenic pistachio in food products.
Several phage display biopanning strategies were evaluated to screen the human-based domain antibody library (dAb) in search for pistachio-specific probes. The clone producing the PVF4 phage-dAb was finally selected, and it does not cross-react with cashew despite the phylogenetic proximity with pistachio.
Western blot and matrix-assisted laser desorption/ionization tandem mass spectrometry (MALDI-TOF/TOF) analysis demonstrated that this clone recognised a unique band of ∼22 kDa related to the basic subunit of pistachio 11S globulin (allergen Pis v 2).
The PVF4 phage-dAb allowed detection of pistachio in a food matrix with a limit of detection (LOD) of 3983 mg kg-1 in an indirect phage-enzyme-linked immunosorbent assay (ELISA). The ELISA method developed was used to assess applicability of the PVF4 phage-dAb for analysis of 77 commercial food products.
 MOUSE ANTI HUMAN CD163 |
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| MBS212351-02mg | MyBiosource | 0.2mg | EUR 550 |
MOUSE ANTI HUMAN CD163 |
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| MBS212351-5x02mg | MyBiosource | 5x0.2mg | EUR 2295 |
 human anti-human CD163 mAb |
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| E4A09C49 | EnoGene | 50ug | EUR 275 |
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Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody |
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 Mouse Anti Human CD163: RPE |
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| MBS225636-100Tests | MyBiosource | 100Tests | EUR 520 |
Mouse Anti Human CD163: RPE |
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| MBS225636-5x100Tests | MyBiosource | 5x100Tests | EUR 2160 |
 MOUSE ANTI HUMAN CD163: FITC |
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| MBS225948-01mg | MyBiosource | 0.1mg | EUR 435 |
MOUSE ANTI HUMAN CD163: FITC |
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| MBS225948-5x01mg | MyBiosource | 5x0.1mg | EUR 1785 |
ISA kit) Human CD163 antigen(CD163)ISA kit |
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| GA-E0263HM-48T | GenAsia Biotech | 48T | EUR 346.8 |
Human CD163 antigen(CD163)ISA kit |
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| GA-E0263HM-48Tests | GenAsia Biotech | 48 Tests | Ask for price |
Human CD163 antigen(CD163)ISA kit |
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| GA-E0263HM-96T | GenAsia Biotech | 96T | EUR 559.2 |
Human CD163 antigen(CD163)ISA kit |
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| GA-E0263HM-96Tests | GenAsia Biotech | 96 Tests | EUR 388 |
 human CD163 antigen,CD163 LISA kit |
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| 201-12-0247 | Shanghai Sunred Biological Technology | 96 tests | EUR 528 |
|
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids. |
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 Human CD163 antigen,CD163 ELISA KIT |
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| E0246Hu-1096T | Jiaxing Korain Biotech Ltd (BT Labs) | 10*96T | EUR 4122 |
Human CD163 antigen,CD163 ELISA KIT |
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| E0246Hu-48wells | Jiaxing Korain Biotech Ltd (BT Labs) | 48 wells | EUR 300 |
Human CD163 antigen,CD163 ELISA KIT |
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| E0246Hu-596T | Jiaxing Korain Biotech Ltd (BT Labs) | 5*96T | EUR 2061 |
Human CD163 antigen,CD163 ELISA KIT |
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| E0246Hu-96wells | Jiaxing Korain Biotech Ltd (BT Labs) | 96 wells | EUR 458 |
Human CD163 antigen,CD163 ELISA KIT |
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| JOT-EK0246Hu | Jotbody | 96 wells | Ask for price |
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Description: Human |
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 Human CD163 antigen, CD163 ELISA Kit |
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| MBS165165-10x96StripWells | MyBiosource | 10x96-Strip-Wells | EUR 3460 |
Human CD163 antigen, CD163 ELISA Kit |
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| MBS165165-48StripWells | MyBiosource | 48-Strip-Wells | EUR 285 |
Human CD163 antigen, CD163 ELISA Kit |
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| MBS165165-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 1750 |
Human CD163 antigen, CD163 ELISA Kit |
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| MBS165165-96StripWells | MyBiosource | 96-Strip-Wells | EUR 425 |
 ELISA Kit) Human CD163 Antigen (CD163) ELISA Kit |
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| MBS9353075-10x96StripWells | MyBiosource | 10x96-Strip-Wells | EUR 6725 |
Human CD163 Antigen (CD163) ELISA Kit |
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| MBS9353075-48StripWells | MyBiosource | 48-Strip-Wells | EUR 550 |
Human CD163 Antigen (CD163) ELISA Kit |
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| MBS9353075-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 3420 |
Human CD163 Antigen (CD163) ELISA Kit |
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| MBS9353075-96StripWells | MyBiosource | 96-Strip-Wells | EUR 765 |
 human CD163 antigen,CD163 ELISA kit |
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| YLA0098HU-48T | Shanghai YL Biotech | 48T | Ask for price |
human CD163 antigen,CD163 ELISA kit |
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| YLA0098HU-96T | Shanghai YL Biotech | 96T | Ask for price |
 Human CD163 Antigen, CD163 GENLISA ELISA |
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| KBH0246 | Krishgen | 1 x 96 wells | EUR 286 |
 Mouse Anti Human Cd163 Monoclonal Antibody |
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| CABT-47162MH | Creative Diagnostics | 25 µg | EUR 322.14 |
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Description: Mouse |
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Mouse Anti Human Cd163 Monoclonal Antibody |
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| CABT-47163MH | Creative Diagnostics | 0.2 mg | EUR 659.4 |
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Description: Mouse |
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 Human CD163 antigen-CD163-ISA kit |
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| QY-E05066 | Qayee Biotechnology | 96T | EUR 400 |
 Mouse Anti Human CD163, APC Conjugated mAb |
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| MBS9431893-100Tests | MyBiosource | 100Tests | EUR 520 |
Mouse Anti Human CD163, APC Conjugated mAb |
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| MBS9431893-25Tests | MyBiosource | 25Tests | EUR 275 |
Mouse Anti Human CD163, APC Conjugated mAb |
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| MBS9431893-5x100Tests | MyBiosource | 5x100Tests | EUR 2195 |
-Human CD163 molecule (CD163), transcript variant 2) CD163 (untagged)-Human CD163 molecule (CD163), transcript variant 2 |
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| SC308167 | Origene Technologies GmbH | 10 µg | Ask for price |
-Human CD163 molecule (CD163), transcript variant 1) CD163 (untagged)-Human CD163 molecule (CD163), transcript variant 1 |
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| SC117495 | Origene Technologies GmbH | 10 µg | Ask for price |
 PE anti-human CD163 |
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| E16FHP163-025 | EnoGene | 25 tests | EUR 433.33 |
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Description: Available in various conjugation types. |
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PE anti-human CD163 |
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| E16FHP163-100 | EnoGene | 100 tests | EUR 953.33 |
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Description: Available in various conjugation types. |
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 Anti-Human CD163 PE |
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| MBS696987-100Tests | MyBiosource | 100Tests | EUR 415 |
Anti-Human CD163 PE |
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| MBS696987-25Tests | MyBiosource | 25Tests | EUR 225 |
Anti-Human CD163 PE |
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| MBS696987-5x100Tests | MyBiosource | 5x100Tests | EUR 1820 |
 Anti-Human CD163 Antibody |
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| 101-M291 | ReliaTech | 100 µg | EUR 399 |
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Description: CD163, also known as hemoglobin scavenger receptor, is a type I transmembrane protein expressed exclusively in monocytes and macrophages. It is a scavenger receptor cysteine-rich superfamily (SRCR-SF) protein that contains nine SRCR motifs in its extracellular region. Two alternatively spliced cytoplasmic variants of human CD163 exist. A soluble form of CD163 can also be released by metalloproteinase-mediated shedding of the extracellular domain. CD163 mediates the endocytosis of haptoglobin-hemoglobin complexes. |
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 APC anti-human CD163 |
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| E16FHA163-025 | EnoGene | 25 tests | EUR 520 |
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Description: Available in various conjugation types. |
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APC anti-human CD163 |
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| E16FHA163-100 | EnoGene | 100 tests | EUR 1191.67 |
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Description: Available in various conjugation types. |
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 Anti-Human CD163 APC |
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| MBS696989-100Tests | MyBiosource | 100Tests | EUR 480 |
Anti-Human CD163 APC |
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| MBS696989-25Tests | MyBiosource | 25Tests | EUR 245 |
Anti-Human CD163 APC |
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| MBS696989-5x100Tests | MyBiosource | 5x100Tests | EUR 2115 |
APC anti-human CD163 |
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| MBS9459445-100Tests | MyBiosource | 100Tests | EUR 655 |
APC anti-human CD163 |
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| MBS9459445-25Tests | MyBiosource | 25Tests | EUR 355 |
APC anti-human CD163 |
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| MBS9459445-5x100Tests | MyBiosource | 5x100Tests | EUR 2790 |
 FITC anti-human CD163 |
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| E16FHF163-025 | EnoGene | 25 tests | EUR 520 |
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Description: Available in various conjugation types. |
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FITC anti-human CD163 |
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| E16FHF163-100 | EnoGene | 100 tests | EUR 1191.67 |
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Description: Available in various conjugation types. |
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 Biotin anti-human CD163 |
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| E16FHB163-025U | EnoGene | 25 μg | EUR 346.67 |
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Description: Available in various conjugation types. |
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Biotin anti-human CD163 |
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| E16FHB163-100U | EnoGene | 100 μg | EUR 780 |
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Description: Available in various conjugation types. |
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 Purified anti-human CD163 |
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| E16FHU163-100U | EnoGene | 100 μg | EUR 433.33 |
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Description: Available in various conjugation types. |
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 - Human CD163 molecule (CD163), transcript variant 2) CD163 (GFP-tagged) - Human CD163 molecule (CD163), transcript variant 2 |
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| RG212754 | Origene Technologies GmbH | 10 µg | Ask for price |
 - Human CD163 molecule (CD163), transcript variant 1) CD163 (GFP-tagged) - Human CD163 molecule (CD163), transcript variant 1 |
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| RG208557 | Origene Technologies GmbH | 10 µg | Ask for price |
 Rabbit Anti Human CD163 Monoclonal Clone ABCC-3 |
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| IRBAHUCD163ABCC3C100UL | Innovative research | each | EUR 496 |
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Description: Rabbit Anti Human CD163 Monoclonal Clone ABCC-3 |
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 Recombinant Human CD163/CD163 Protein, His Tag |
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| E40KMH346 | EnoGene | 20ug | EUR 495 |
 Human CD163 siRNA |
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| 20-abx910931 | Abbexa |
|
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 Human CD163 Protein |
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| E40KMPH6242 | EnoGene | 20ug | EUR 495 |
-Human CD163 molecule (CD163), transcript variant 1) CD163 (Myc-DDK-tagged)-Human CD163 molecule (CD163), transcript variant 1 |
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| RC208557 | Origene Technologies GmbH | 10 µg | Ask for price |
-Human CD163 molecule (CD163), transcript variant 2) CD163 (Myc-DDK-tagged)-Human CD163 molecule (CD163), transcript variant 2 |
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| RC212754 | Origene Technologies GmbH | 10 µg | Ask for price |
 Recombinant Human CD163 |
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| MBS147285-0001mg | MyBiosource | 0.001mg | EUR 240 |
Recombinant Human CD163 |
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| MBS147285-0005mg | MyBiosource | 0.005mg | EUR 310 |
Recombinant Human CD163 |
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| MBS147285-005mg | MyBiosource | 0.05mg | EUR 1360 |
Recombinant Human CD163 |
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| MBS147285-5x005mg | MyBiosource | 5x0.05mg | EUR 5800 |
) CD163 siRNA (Human) |
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| MBS8229683-15nmol | MyBiosource | 15nmol | EUR 405 |
CD163 siRNA (Human) |
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| MBS8229683-30nmol | MyBiosource | 30nmol | EUR 565 |
CD163 siRNA (Human) |
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| MBS8229683-5x30nmol | MyBiosource | 5x30nmol | EUR 2450 |
) Anti-Human CD163 Antibody (M130) |
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| MBS1564482-01mg | MyBiosource | 0.1mg | EUR 340 |
Anti-Human CD163 Antibody (M130) |
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| MBS1564482-5x01mg | MyBiosource | 5x0.1mg | EUR 1235 |
) Anti-Human CD163 Antibody (AB101) |
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| MBS1570008-01mg | MyBiosource | 0.1mg | EUR 405 |
Anti-Human CD163 Antibody (AB101) |
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| MBS1570008-5x01mg | MyBiosource | 5x0.1mg | EUR 1520 |
 PE-Cy7 anti-human CD163 |
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| E16FHN163-025 | EnoGene | 25 tests | EUR 650 |
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Description: Available in various conjugation types. |
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PE-Cy7 anti-human CD163 |
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| E16FHN163-100 | EnoGene | 100 tests | EUR 1408.33 |
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Description: Available in various conjugation types. |
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 APC-Cy7 anti-human CD163 |
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| E16FHO163-025 | EnoGene | 25 tests | EUR 650 |
|
Description: Available in various conjugation types. |
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APC-Cy7 anti-human CD163 |
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| E16FHO163-100 | EnoGene | 100 tests | EUR 1408.33 |
|
Description: Available in various conjugation types. |
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 PerCP-Cy5.5 anti-human CD163 |
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| E16FHS163-025 | EnoGene | 25 tests | EUR 541.67 |
|
Description: Available in various conjugation types. |
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PerCP-Cy5.5 anti-human CD163 |
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| E16FHS163-100 | EnoGene | 100 tests | EUR 1278.33 |
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Description: Available in various conjugation types. |
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 Anti-Human CD163 PE-Cyanine7 |
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| MBS696988-100Tests | MyBiosource | 100Tests | EUR 505 |
Anti-Human CD163 PE-Cyanine7 |
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| MBS696988-25Tests | MyBiosource | 25Tests | EUR 270 |
Anti-Human CD163 PE-Cyanine7 |
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| MBS696988-5x100Tests | MyBiosource | 5x100Tests | EUR 2220 |
 Mouse Monoclonal anti-human CD163 |
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| hAP-0161 | Angio Proteomie | 100ug | EUR 250 |
Mouse Monoclonal anti-human CD163 |
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| hAP-0161A | Angio Proteomie | 50ug | EUR 400 |
 Human CD163, Fc Tag |
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| E24PHA282 | EnoGene | 50 μg | EUR 250 |
 Human, Rat, Mouse CD163 antibody |
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| P0405 | Omnimabs | 100ul | EUR 150 |
 Human CD163, His Tag |
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| E24PHA281 | EnoGene | 50 μg | EUR 250 |
 Human CD163 ELISA Kit |
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| LF-EK50890 | Abfrontier | 1×96T | EUR 777.6 |
 Human CD163 ELISA Kit |
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| EK5296 | SAB | 96 tests | EUR 599 |
Human CD163 ELISA Kit |
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| GWB-SKR207 | GenWay Biotech | 96 Tests | Ask for price |
Human CD163 ELISA Kit |
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| ELA-E1726h | Lifescience Market | 96 Tests | EUR 988.8 |
Human CD163 ELISA Kit |
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| MBS3800092-10x96StripWells | MyBiosource | 10x96-Strip-Wells | EUR 6725 |
Human CD163 ELISA Kit |
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| MBS3800092-48StripWells | MyBiosource | 48-Strip-Wells | EUR 550 |
Human CD163 ELISA Kit |
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| MBS3800092-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 3420 |
Human CD163 ELISA Kit |
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| MBS3800092-96StripWells | MyBiosource | 96-Strip-Wells | EUR 765 |
Human CD163 ELISA Kit |
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| MBS824474-5x96Tests | MyBiosource | 5x96Tests | EUR 2880 |
Human CD163 ELISA Kit |
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| MBS824474-96Tests | MyBiosource | 96Tests | EUR 645 |
 Human CD163 shRNA Plasmid |
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| 20-abx956176 | Abbexa |
|
|
 Human CD163 Protein Lysate |
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| MBS138999-002mg | MyBiosource | 0.02mg | EUR 365 |
Human CD163 Protein Lysate |
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| MBS138999-5x002mg | MyBiosource | 5x0.02mg | EUR 1410 |
 Human CD163 Recombinant Protein |
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| PROTQ86VB7 | BosterBio | 10ug | EUR 350 |
|
Description: Human CD163 Recombinant Protein expressed in Baculovirus with His-tag. Sequence domain: 42-1050aa. Application(s): SDS-PAGE. Endotoxin: < 1 EU per 1ug of protein (determined by LAL method). |
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 Recombinant Human CD163 Protein |
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| RP02295 | Abclonal | 500μg | EUR 244.43 |
 ELISA Kit) CD163 (Human) ELISA Kit |
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| EKA52023-5x96T | Biomatik Corporation | 5x96T | Ask for price |
CD163 (Human) ELISA Kit |
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| EKA52023-96T | Biomatik Corporation | 96T | EUR 446.9 |
 Human CellExp? CD163, Human recombinant |
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| 9226-10 | Biovision | each | EUR 366 |
Human CellExp? CD163, Human recombinant |
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| 9226-50 | Biovision | each | EUR 927.6 |
 anti- CD163/M130 Antibody |
|||
| FNab09771 | FN Test | 100µg | EUR 658.5 |
|
Description: Antibody raised against CD163/M130 |
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